Agenda

Day 1 — Tuesday, Nov. 3

*All times PST

Morning Sessions

8:30 a.m. Registration
Welcome & Coffee
Building 180, Room 101
9:00 a.m. Workshop Opening
Nick Benardini, Planetary Protection Officer, NASA Headquarters
Moogega Cooper, Planetary Protection Engineer, JPL
Marty Herman, Manager, Office and Safety and Mission Success, JPL
9:30 a.m. Keynote — Assuring the Journey: Merging Complex Data Streams into Clear, Objective Safety Requirements
Nathan Vassberg, Chief, NASA Safety and Mission Assurance
10:00 a.m. Break
10:20 a.m. Roadmap / Current Progress — Opening
Lisa Guan, Planetary Protection Engineer, JPL
Benjamin Andrews, ESA

This session will give an overview of the progress made since the previous workshop.
10:40 a.m. Agency Progress Reports

International SOP for Metagenomics · Computational Tools · International & Cross-Discipline Collaboration · Metagenomics for PRA  
NASA — Nick Benardini, Planetary Protection Officer, NASA Headquarters
ESA — Silvio Sinibaldi, Planetary Protection Officer, ESA
JAXA — Takashi Yamazaki, Professor, Planetary Protection Officer, JAXA
COSPAR — Karen Olsson-Francis, Professor, The Open University

This session will cover the agency-specific progress made since the previous workshop.

11:40 a.m. Current Status / State of the Art — Opening
Moogega Cooper, Planetary Protection Engineer, JPL
Rodrigo Almeida, ESA
Sandra Ortega Ugalde, ESA
11:50 a.m. State of the Art Presentations

JPL Low-Biomass ddPCR/Seq
Moogega Cooper, Planetary Protection Engineer, JPL
Lisa Guan, Planetary Protection Engineer, JPL

Low-Biomass Bioinformatics Pipeline
Amanda M. Saravia-Butler, Science Lead, GeneLab, NASA Open Science Data Repository, Ames Research Center

CRISPR-Enhanced Next Generation Sequencing of Metagenomics: Motivation and Applications
Nicholas J. Schork, Professor, Translational Genomics Research Institute
12:30 p.m. Lunch

Afternoon Sessions

1:30 p.m. State of the Art Presentations — Block 2

Metagenomics for Human Space Flight: From ISS to Artemis 

Aaron Regberg, Planetary Protection Officer, Johnson Space Center

Data and Sample Repositories
Jonathan Galazka, OSDR Project Manager, Ames Research Center
Jessica Lee, Research Scientist / Curation Lead, Space Microbial Culture Collection, Ames Research Center

Microbiome Data Submission and Genome Catalogue Construction for Spacecraft Cleanroom Environments
Tatiana Gurbich, ENA EBI
Joana Paupério, ENA EBI
2:00 p.m. State of the Art Presentations — Block 3

Computational Modeling, ddPCR & Ideal Score
Michael DiNicola, Senior Systems Engineer, JPL

Capillary Electrophoresis Instrumentation for In Situ Chemical Analysis and Identification of Biosignatures
Maria Fernanda Mora, Chemical Analysis and Life Detection, JPL

Metagenomics in Commercial Space
Emily Seto, Senior Manager of Planetary Protection and Contamination Control and Research, Honeybee Robotics
2:45 p.m. Transition to Von Karman
2:50 p.m. Break
3:05 p.m. Panel 1 — Metagenomics Panel (Q&A / Dialogue)
Moderators: 
Moogega Cooper, Planetary Protection Engineer, JPL
Sandra Ortega Ugalde, ESA

Chelsi Cassilly, Planetary Protection Engineer, Marshall Space Flight Center
Lisa Guan, Planetary Protection Engineer, JPL
Aaron Regberg, Planetary Protection Officer, Johnson Space Center
Maria Fernanda Mora, Chemical Analysis and Life Detection, JPL
Emily Seto, Senior Manager of Planetary Protection and Contamination Control and Research, Honeybee Robotics
Karen Olsson-Francis, Professor, The Open University
3:50 p.m.

Panel 2 — Computational Panel (Q&A / Dialogue)
Moderators: 
Moogega Cooper, Planetary Protection Engineer, JPL
Rodrigo Almeida, ESA

Amanda M. Saravia-Butler, Science Lead, GeneLab, NASA Open Science Data Repository, Ames Research Center
Jon Galazka, OSDR Project Manager, Ames Research Center
Jessica Lee, Research Scientist / Curation Lead, Space Microbial Culture Collection, Ames Research Center
Michael DiNicola, Senior Systems Engineer, JPL

4:35 p.m. Day 1 Closing Remarks
5:15 p.m. Social Hour — King's Row Gastropub

Day 2 — Wednesday, Nov. 4

Morning Sessions

8:30 a.m. Welcome
Registration & Coffee
Building 180, Room 101
9:00 a.m. Standardized Practices — Opening
Christina Davis, Postdoctoral Researcher, McGill University
Samrawit Gebre, BPS Data Officer, Ames Research Center
9:10 a.m.

Optimizing Metagenomics Operations for Spaceflight: An Engineer / Project Manager’s Reality Check
Moogega Cooper, Planetary Protection Engineer, JPL
Lisa Guan, Planetary Protection Engineer, JPL

This session will explore the operational impacts and cost considerations of implementing standardized practices for metagenomics assays, highlighting how engineering and project management perspectives drive efficiency and resource optimization in spaceflight applications. How and where should we add metagenomics in the operations.

9:40 a.m. Standardized Practices in Other Disciplines

This session examines how diverse fields—including ancient DNA research, clinical and regulatory frameworks, Antarctic sub-glacier exploration, and NIST—have established standard practices, offering insights into cross-disciplinary approaches for developing robust protocols.

9:40 a.m. — The Journey to Clean Access Sampling of Antarctic Subglacial Lakes
Alex Michaud, Microbial Biogeochemist, The Ohio State University

10:00 a.m. — Documentary Standards 101
Stephanie Servetas, Supervisory Microbiologist, National Institute of Standards and Technology

10:20 a.m. Break
10:40 a.m. Quality Assurance in Metagenomics — Part 1

This session presents best practices and recommendations for sample preparation, controls, and threshold setting, featuring JPL’s controls and threshold framework, MSFC pilot lessons learned, and approaches to spike-in standards, with a focus on quality checkpoints and outputs to ensure robust metagenomics assay results.

10:40 a.m. — Standardizing Advanced Biotechnology Techniques for Planetary Protection
Lisa Guan, Planetary Protection Engineer, JPL

10:55 a.m. — Using Metagenomics to Characterize NASA Cleanrooms: Lessons Learned from a Multi-Center Study
Aaron Regberg, Planetary Protection Officer, Johnson Space Center

11:10 a.m. — Developments and Needs for a Modular Molecular Workflow in PP 
Stefan Green, Director, Core Laboratory Services, Rush University

11:25 a.m. — ESA Initial Standardization
Silvio Sinibaldi, Planetary Protection Officer, ESA

11:40 a.m. Quality Assurance in Metagenomics — Part 2

This session addresses the critical bioinformatics and sequencing challenges in metagenomics, including astrobiology background requirements, the tradeoffs between long and short read technologies, and examples of operational bioinformatics pipelines.

11:40 a.m. — DNA Resilience Under Radiation: Implications for Planetary Protection Practices
Maria-Paz Zorzano, Senior Researcher, Centro de Astrobiologia (CAB), INTA-CSIC, Spain

11:55 a.m. — Critical Noise Reduction in Low Biomass Planetary Protection Metagenomics
Scott Tighe, Senior Research Associate, University of Vermont

12:10 p.m. — Metagenomics Workflows in Spaceflight-Related Environments: A Decade-Long Optimization Process
Alexander Mahnert, Medical University of Graz, Diagnostic & Research Institute of Hygiene, Microbiology and Environmental Medicine

12:20 p.m. Lunch
Leave belongings in B180 before tour

Afternoon Sessions

1:15 p.m. JPL Tour
Guided tour of JPL facilities; ends back at B180, then transition to Von Karman 
2:35 p.m. JPL tour concludes at B180. Collect belongings and transition from B180 to Von Karman.
2:50 p.m. Breakout — How Do We Make It Adoptable?
Moderators:
Christina Davis, Postdoctoral Researcher, McGill University
Samrawit Gebre, BPS Data Officer, Ames Research Center

This session will define actionable steps for making metagenomics workflows broadly adoptable, focusing on immediate and long-term plans for threshold and quality control at every stage, optimal use of controls and spike-ins, public protocol sharing, standardized QC requirements, reference datasets, quality scoring, contamination management, platform comparisons, and collaborative development of quantifiable thresholds and interoperable standards for both low and ultra-low biomass samples.

4:35 p.m. Reconvene: Report-Out from Breakout
Breakout Leads
4:50 p.m. Wrap-Up and Day 2 Close

Day 3 — Thursday, Nov. 5

Morning Sessions

8:30 a.m. Welcome
Registration & Coffee
Building 180, Room 101
8:40 a.m. Technology Needs — Opening

Alexander Mahnert, Medical University of Graz, Diagnostic & Research Institute of Hygiene, Microbiology and Environmental Medicine
Ceth Parker, Staff Scientist, JPL

This session will provide us with insight into the developments that will be necessary in the field of metagenomics in space exploration. It will feature presentations from both the academic and industrial sectors, aimed at reaching a consensus between the two sectors regarding the next priorities and steps toward achieving future goals. The academic presentations are intended to broaden our horizons, while the industry presentations will provide insights into current solutions and upcoming developments.

8:50 a.m. Technology Talks — Academia

Built Environment: Microbiology On the Edge of Survival
Jack Gilbert, Professor, UC San Diego

En Garde! Battling Antimicrobial Resistance in Built Environments with Microbial Biocontrol  
Kat Furtado, UC San Diego

Leveraging Nextflow to Implement Standardized and Portable Metagenomics Processing Pipelines 
Barbara Novak, GeneLab Data Processing Lead, NASA OSDR/GeneLab; Blue Marble Space
9:40 a.m. Break
10:00 a.m. Technology Talks — Industry

10:00 a.m. — Advancing Low-Biomass Metagenomics: New Tools and Standards for Planetary Protection
Kristopher Locken, Molecular Microbiologist, Zymo Research

10:15 a.m. — Universal Sequencing: Single- and Double-Stranded RNA and DNA, from Bacteria, Viruses, and Fungi in a Unified Workflow
Dominic O'Neil, R&D Director of Microbiome Product Development, QIAGEN

10:30 a.m. — Expanding Your Metagenomic Toolkit with Oxford Nanopore Sequencing
Candace Williams and/or Jay Mehta (ONT)

10:45 a.m. — Mapping the Unknown Activities of Life: Structure-Based AI for Metagenomic Dark Matter
Christian Gruber, CEO, Innophore

11:00 a.m. — Trace Biomass Detection with ddPCR
Katie Orban, Bio-Rad (dPCR)

11:15 a.m. — Potential Application of Primary Template-Directed Amplification to Detect Low-Biomass DNA During Space Exploration
Chuck Gawad, BioSkryb

11:30 a.m. — Potential Applications of Sequencing By Expansion (SBX) Technology for Planetary Protection Applications
Sam Modlin, Roche
11:45 a.m. Lunch
Transition to Von Karman

Afternoon Sessions

12:35 p.m. Transition to Von Karman
12:40 p.m.

12:40 p.m. — High Technology Readiness Level (TRL) Astrobiology Technology Used for Planetary Protection Applications
Ceth Parker, Staff Scientist, JPL

12:50 p.m. — Technology Panel — Needs & Solutions: Consensus on Priorities. Technology Session Wrap-Up
Moderators:
Alexander Mahnert, Medical University of Graz, Diagnostic & Research Institute of Hygiene, Microbiology and Environmental Medicine
Ceth Parker, Staff Scientist, JPL

All Technology Session Participants

1:45 p.m. Safety Critical Decision Making — Opening
Moderators: 
Nick Benardini, Planetary Protection Officer, NASA Headquarters
Silvio Sinibaldi, Planetary Protection Officer, ESA
Erin Lalime, Deputy Planetary Protection Officer, NASA Headquarters
Andy Spry, Subject Matter Expert, Planetary Protection, BQMI
2:00 p.m. Safety Critical Decision Making — Talks & Discussion:
Planetary Protection, Crew Health, Threshold-Based Decision Frameworks, Regulatory Alignment

Metagenomics in Industry Decision Making
Stephanie Servetas, Supervisory Microbiologist, National Institute of Standards and Technology

Functional Metagenomics in Risk Categorization
Erin Lalime, Deputy Planetary Protection Officer, NASA Headquarters
2:45 p.m. Break
3:05 p.m. Safety Critical Decision Making — Talks & Discussion:
Planetary Protection, Crew Health, Threshold-Based Decision Frameworks, Regulatory Alignment

ESA Icy Worlds JUICE Use Case
Alexander Mahnert, Medical University of Graz, Diagnostic & Research Institute of Hygiene, Microbiology and Environmental Medicine

NASA Planetary Protection Case Study and Development
Moogega Cooper, Planetary Protection Engineer, JPL
Lisa Guan, Planetary Protection Engineer, JPL

Enabling Risk-Informed Planetary Protection Through a Genomics-Driven Probabilistic Approach to Forward Contamination
Benjamin Andrews, ESA
Silvio Sinibaldi, Planetary Protection Officer, ESA
Karen Olsson-Francis, Professor, The Open University
Alexander Mahnert, Medical University of Graz, Diagnostic & Research Institute of Hygiene, Microbiology and Environmental Medicine

5:00 p.m. Day 3 Close

Day 4 — Friday, Nov. 6

8:30 a.m. Dark Matter — Opening & Framing

Summary of Microbial Dark Matter Symposium
Haley Sapers, Assistant Professor, Northern Arizona University
Kristopher Locken, Molecular Microbiologist, Zymo Research

Quantitative Uncertainty Modeling and Microbial Dark Matter
Jared Broddrick, Systems Biologist, Ames Research Center
9:20 a.m. Dark Matter — Talks, Panel and Discussion
Moderators:
Haley Sapers, Assistant Professor, Northern Arizona University
Jared Broddrick, Systems Biologist, Ames Research Center
10:20 a.m. Break
10:30 a.m. Roadmap Breakout — What Changes Are Needed? Reconvene & Synthesis
Moderators:
Lisa Guan, Planetary Protection Engineer, JPL
Benjamin Andrews, ESA

Breakout Topic: 
What roadblocks remain for our roadmap? What are potential solutions?

10:30 - 11:10 a.m. — Breakout
11:10 - 11:30 a.m. — Reconvene & Synthesis

11:30 a.m. Working Lunch
12:15 p.m. Closing Session — Summary of Each Session:
Roadmap · State of Art · Standards · Safety Critical · Dark Matter · Tech Needs
Session Chairs

Action Items & Accountability

Closing Remarks & Adjourn
Nick Benardini, Planetary Protection Officer, NASA Headquarters
2:00 p.m. Workshop Concludes