Agenda

Tuesday, Nov. 3

*All times PST

Morning Sessions

9:00 a.m. Workshop Opening
Nick Benardini, Planetary Protection Officer, NASA Headquarters
Moogega Cooper, Planetary Protection Engineer, JPL
9:30 a.m. Keynote — Assuring the Journey: Merging Complex Data Streams into Clear, Objective Safety Requirements
Nathan Vassberg, Chief, NASA Safety and Mission Assurance
10:00 a.m. Break
10:20 a.m. Roadmap / Current Progress — Opening
Lisa Guan, Planetary Protection Engineer, JPL
Benjamin Andrews, ESA

This session will give an overview of the progress made since the previous workshop
10:40 a.m. Agency Progress Reports

International SOP for Metagenomics · Computational Tools · International & Cross-discipline Collaboration · Metagenomics for PRA  
NASA — Nick Benardini, Planetary Protection Officer, NASA Headquarters
ESA — Silvio Sinibaldi, Planetary Protection Officer, ESA
JAXA — Takashi Yamazaki, Professor, JAXA
UKSA — Karen Francis, Professor, The Open University

This session will cover the agency-specific progress made since the previous workshop

11:40 a.m. Current Status / State of the Art — Opening
Moogega Cooper, Planetary Protection Engineer, JPL
Rodrigo Almeida, ESA
Sandra Ortega Ugalde, ESA
11:50 a.m. State of the Art Presentations

JPL Low-Biomass ddPCR/Seq
Moogega Cooper, Planetary Protection Engineer, JPL
Lisa Guan, Planetary Protection Engineer, JPL

Low-Biomass Bioinformatics Pipeline
Amanda M. Saravia-Butler, Science Lead, GeneLab, NASA Open Science Data Repository, Ames Research Center
Olabiyi A. Obayomi, Bioinformatics Programmer and Developer, Amentum Inc., Ames Research Center

CRISPR-Enhanced Metagenomic NGS
Nicholas J. Schork, Professor, Translational Genomics Research Institute
12:30 p.m. Lunch

Afternoon Sessions

1:30 p.m. State of the Art Presentations — Block 2

ISS / Human Spaceflight Metagenomics (JSC)

Sarah Wallace 
Aaron Regberg, Space Scientist, Johnson Space Center

Repository: OSDR / SMCC / MGnify
Jon Galazka, OSDR Project Manager, Ames Research Center
Jessica Lee, Research Scientist / Curation Lead, Space Microbial Culture Collection, Ames Research Center

Repository: EBI & European Partners
EBI Rep
2:00 p.m. State of the Art Presentations — Block 3

Computational Modeling, ddPCR & Ideal Score
Michael DiNicola, Senior Systems Engineer, JPL

Astrobiology State of the Art / Life Detection
Peter Willis

Metagenomics in the Private Sector (Honeybee Robotics)
Emily Seto, Senior Manager of Planetary Protection and Contamination Control and Research, Honeybee Robotics
2:45 p.m. Break
3:00 p.m. Transition to Von Karman
3:05 p.m. Panel 1 — Metagenomics Panel (Q&A / Dialogue)
Moderators: 
Moogega Cooper, Planetary Protection Engineer, JPL
Sandra Ortega Ugalde, ESA

Chelsi Cassilly, Planetary Protection Engineer, Marshall Space Flight Center
Ceth Parker, Staff Scientist, JPL
Lisa Guan, Planetary Protection Engineer, JPL
Sarah Wallace
Aaron Regberg, Space Scientist, Johnson Space Center
Silvio Sinibaldi, Planetary Protection Officer, ESA
Emily Seto, Senior Manager of Planetary Protection and Contamination Control and Research, Honeybee Robotics
Karen Francis, Professor, The Open University
3:50 p.m.

Panel 2 — Computational Panel (Q&A / Dialogue)
Moderators: 
Moogega Cooper, Planetary Protection Engineer, JPL
Rodrigo Almeida, ESA

Amanda M. Saravia-Butler, Science Lead, GeneLab, NASA Open Science Data Repository, Ames Research Center
Olabiyi A. Obayomi, Bioinformatics Programmer and Developer, Amentum Inc., Ames Research Center
Jon Galazka, OSDR Project Manager, Ames Research Center
Jessica Lee, Research Scientist / Curation Lead, Space Microbial Culture Collection, Ames Research Center
EBI Rep
Michael DiNicola, Senior Systems Engineer, JPL

4:35 p.m. Day 1 Close
5:15 p.m. Social Hour — King's Row Gastropub

Wednesday, Nov. 4

Morning Sessions

9:00 a.m. Standardized Practices — Opening
Christina Davis, Postdoctoral Researcher, McGill University
Samrawit Gebre, BPS Data Officer, Ames Research Center
9:10 a.m. Engineer / PM Perspective

This session will explore the operational impacts and cost considerations of implementing standardized practices for metagenomics assays, highlighting how engineering and project management perspectives drive efficiency and resource optimization in spaceflight applications.

9:30 a.m. Standardized Practices in Other Disciplines

This session examines how diverse fields—including ancient DNA research, clinical and regulatory frameworks, Antarctic sub-glacier exploration, and NIST—have established standard practices, offering insights into cross-disciplinary approaches for developing robust protocols.

Ancient DNA

Clinical / FDA / CDC AMD

Antarctica Sub-Glacier Project
Alex Michaud, Microbial Biogeochemist, The Ohio State University

NIST
Stephanie Servetas, Supervisory Microbiologist, National Institute of Standards and Technology

10:30 a.m. Break
10:50 a.m. Quality Assurance in Metagenomics: Sample Preparation, Controls and Thresholds

This session presents best practices and recommendations for sample preparation, controls, and threshold setting, featuring JPL’s controls and threshold framework, MSFC pilot lessons learned, and approaches to spike-in standards, with a focus on quality checkpoints and outputs to ensure robust metagenomics assay results

JPL Controls and Threshold Framework
Lisa Guan, Planetary Protection Engineer, JPL

MSFC Pilot Lessons Learned
Chelsi Cassilly, Planetary Protection Engineer, Marshall Space Flight Center
Aaron Regberg, Space Scientist, Johnson Space Center

Controls and Spike-in Standards
Stefan Green, Director, Core Laboratory Services, Rush University
Scott Tighe, Research Faculty, University of Vermont

ESA Quality Control

11:35 a.m. Sequencing and Bioinformatics Quality — Ultra Low Biomass

This session addresses the critical bioinformatics and sequencing challenges in metagenomics, including astrobiology background requirements, strategies for spike-in removal and data representation, the tradeoffs between long and short read technologies, and a study from ESA focused on quality control protocols.

Astrobiology Ultra Low Biomass
Maria-Paz Zorzano, Senior Researcher, Centro de Astrobiologia (CAB)

Spike-in Remove and Bioinformatics Pipeline
Alex Michaud, Microbial Biogeochemist, The Ohio State University

Long vs Short Read
Scott Tighe, Research Faculty, University of Vermont

Threshold / Quality
Stefan Green, Director, Core Laboratory Services, Rush University

12:20 p.m. Lunch
Leave belongings in B180 before tour

Afternoon Sessions

1:15 p.m. JPL Tour
Guided tour of JPL facilities; ends back at B180, then transition to Von Karman 
2:35 p.m. JPL tour concludes at B180. Transition from B180 to Von Karman.
2:50 p.m. Breakout — How Do We Make It Adoptable?
Moderators:
Christina Davis, Postdoctoral Researcher, McGill University
Samrawit Gebre, BPS Data Officer, Ames Research Center

This session will define actionable steps for making metagenomics workflows broadly adoptable, focusing on immediate and long-term plans for threshold and quality control at every stage, optimal use of controls and spike-ins, public protocol sharing, standardized QC requirements, reference datasets, quality scoring, contamination management, platform comparisons, and collaborative development of quantifiable thresholds and interoperable standards for both low and ultra-low biomass samples.

4:35 p.m. Reconvene: Report-Out from Breakout
Breakout Leads
4:50 p.m. Wrap-Up and Day 2 Close

Thursday, Nov. 5

Morning Sessions

9:00 a.m. Technology Needs — Opening

Alexandar Mahnert, Medical University of Graz, Diagnostic & Research Institute of Hygiene, Microbiology and Environmental Medicine
Ceth Parker, Staff Scientist, JPL

9:10 a.m. Technology Talks — Academia
Jack Gilbert, Professor, UC San Diego
Kat Furtado
Barbara Novak, GeneLab Data Processing Lead, NASA OSDR/GeneLab; Blue Marble Space
10:00 a.m. Break
10:20 a.m. Technology Talks — Industry

Zymo Research Low-Biomass Kits
Kristopher Locken, Molecular Microbiologist, Zymo Research

Qiagen Sample Prep
Dominic O'Neil, R&D Director of Microbiome Product Development, QIAGEN

Oxford Nanopore
Anna Maria Niewiadomska, Associate Director, Segment Marketing — Micro & Infectious Disease, Oxford Nanopore Technologies

Innophore
Christian Gruber, CEO, Innophore

Bio-Rad dPCR

Bioskryb
Chuck Gawad, BioSkryb

Roche (Low Biomass Sequencing)

Triangle Biotechnology (Low Biomass DNA Extraction)
11:40 a.m. Lunch
Transition to Von Karman

Afternoon Sessions

12:35 p.m. Transition to Von Karman
12:40 p.m. Technology Panel — Needs & Solutions: Consensus on Priorities
Technology Session Wrap-Up
Moderators:
Alexandar Mahnert, Medical University of Graz, Diagnostic & Research Institute of Hygiene, Microbiology and Environmental Medicine
Ceth Parker, Staff Scientist, JPL

All Technology Session Participants
1:45 p.m. Safety Critical Decision Making — Opening
Moderators: 
Nick Benardini, Planetary Protection Officer, NASA Headquarters
Silvio Sinibaldi, Planetary Protection Officer, ESA
Erin Lalime, Deputy Planetary Protection Officer, NASA Headquarters
Andy Spry, Subject Matter Expert, Planetary Protection, BQMI
2:00 p.m. Safety Critical Decision Making — Talks & Discussion:
Planetary Protection, Crew Health, Threshold-Based Decision Frameworks, Regulatory Alignment

Metagenomics in Industry Decision Making
Stephanie Servetas, Supervisory Microbiologist, National Institute of Standards and Technology

Food and Safety Compliance
Rob Donodrio
2:45 p.m. Break
3:05 p.m. Safety Critical Decision Making — Talks & Discussion:
Planetary Protection, Crew Health, Threshold-Based Decision Frameworks, Regulatory Alignment

ESA Icy Worlds JUICE Use Case
Alexandar Mahnert, Medical University of Graz, Diagnostic & Research Institute of Hygiene, Microbiology and Environmental Medicine

NASA Planetary Protection Case Study and Development
Moogega Cooper, Planetary Protection Engineer, JPL
Lisa Guan, Planetary Protection Engineer, JPL
5:00 p.m. Day 3 Close

Friday, Nov. 6

8:30 a.m. Dark Matter — Opening & Framing

Summary of Microbial Dark Matter Symposium
Haley Sapers, Assistant Professor, Northern Arizona University
Kristopher Locken, Molecular Microbiologist, Zymo Research

Quantitative Uncertainty Modeling and Microbial Dark Matter
Jared Broddrick, Systems Biologist, Ames Research Center
9:20 a.m. Dark Matter — Talks, Panel and Discussion
Moderators:
Haley Sapers, Assistant Professor, Northern Arizona University
Jared Broddrick, Systems Biologist, Ames Research Center
10:20 a.m. Break
10:30 a.m. Roadmap Breakout — What Changes Are Needed? Reconvene and Synthesis
Moderators:
Lisa Guan, Planetary Protection Engineer, JPL
Benjamin Andrews, ESA

Breakout Topic: 
What roadblocks remain for our roadmap? What are potential solutions?

11:30 a.m. Working Lunch
12:15 p.m. Closing Session — Summary of Each Session
Roadmap · State of Art · Standards · Safety Critical · Dark Matter · Tech Needs
Session Chairs

Action Items & Accountability

Closing Remarks & Adjourn
Nick Benardini, Planetary Protection Officer, NASA Headquarters
2:00 p.m. Workshop Concludes